LOCUS       CHPF2-pGEn2             7536 bp    DNA     circular     20-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6164..6705
                     /note="WPRE"
     misc_recomb     6126..6148
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(6712..6926)
                     /note="BGH pA"
     misc_feature    complement(7014..7143)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..6119
                     /note="OPTIMIZED CHPF2 coding region"
     vector          join(6134..7536,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..6133
                     /source="CHPF2 pDONR"
                     /type="Entry Clone"
     source          1..7536
                     /dnas_title="CHPF2-pGEn2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc AGAGTGTCTT
     3901 GGATCCAGGG CGAGGGCGAA GATCCTTGTG TGGAAGCTGT GGGAGAAAGA GGCGGCCCTC
     3961 AGAATCCTGA TAGCAGAGCC AGACTGGACC AGAGCGACGA GGACTTCAAG CCCAGAATCG
     4021 TGCCCTACTA CAGGGACCCC AACAAGCCCT ACAAGAAGGT GCTGAGGACC CGGTACATCC
     4081 AGACAGAGCT GGGCAGCAGA GAAAGACTGC TGGTGGCCGT GCTGACCTCT AGAGCCACAC
     4141 TGTCTACACT GGCCGTGGCT GTGAATAGAA CCGTGGCCCA CCATTTCCCC CGGCTGCTGT
     4201 ACTTTACTGG ACAGAGAGGT GCTAGAGCCC CTGCCGGAAT GCAGGTCGTG TCTCATGGCG
     4261 ACGAAAGACC TGCCTGGCTG ATGAGCGAGA CACTGAGACA TCTGCACACC CACTTTGGCG
     4321 CCGACTACGA CTGGTTCTTC ATTATGCAGG ACGACACCTA CGTGCAGGCC CCTAGACTGG
     4381 CTGCTCTGGC CGGACATCTG AGCATCAACC AGGATCTGTA CCTGGGCAGA GCCGAGGAAT
     4441 TCATTGGAGC CGGCGAGCAG GCCAGATACT GTCACGGTGG ATTTGGCTAC CTGCTGAGCA
     4501 GAAGCCTGCT GCTGAGACTG AGGCCACACC TGGATGGCTG TAGAGGCGAT ATCCTGAGCG
     4561 CCAGACCTGA CGAGTGGCTG GGAAGATGTC TGATCGATTC TCTGGGCGTG GGCTGTGTGT
     4621 CACAGCACCA GGGACAGCAG TACAGAAGCT TCGAGCTGGC CAAGAACCGG GACCCTGAGA
     4681 AAGAAGGATC CAGCGCCTTT CTGTCCGCCT TTGCCGTGCA TCCTGTGTCC GAGGGCACAC
     4741 TGATGTACAG ACTGCACAAG CGGTTCTCTG CCCTGGAACT GGAAAGAGCC TACAGCGAGA
     4801 TTGAGCAGCT GCAGGCTCAG ATCAGAAACC TGACAGTGCT GACACCTGAA GGCGAGGCTG
     4861 GACTGTCTTG GCCTGTGGGA CTGCCTGCCC CTTTTACCCC TCACAGCAGA TTTGAGGTGC
     4921 TGGGCTGGGA CTACTTCACC GAGCAGCACA CCTTTAGCTG TGCCGATGGC GCTCCTAAGT
     4981 GTCCACTGCA GGGCGCTTCT AGAGCTGACG TGGGAGATGC TCTGGAAACC GCACTGGAAC
     5041 AGCTGAACAG AAGATACCAG CCAAGACTGA GATTCCAGAA GCAGAGGCTG CTGAACGGCT
     5101 ATCGGAGATT CGATCCTGCC AGAGGCATGG AGTACACACT GGATCTGCTC CTCGAGTGCG
     5161 TGACCCAGAG AGGACATAGA AGGGCACTGG CCAGAAGAGT GTCACTGCTG AGGCCCCTGT
     5221 CCAGAGTGGA AATCCTGCCT ATGCCTTACG TGACCGAAGC CACCAGAGTG CAGCTGGTGC
     5281 TCCCTCTGCT GGTCGCTGAA GCTGCCGCTG CTCCTGCATT TCTGGAAGCC TTTGCTGCCA
     5341 ACGTGCTGGA ACCTAGAGAA CACGCTCTGC TGACCCTCCT CCTGGTGTAT GGACCAAGAG
     5401 AAGGTGGCAG AGGCGCCCCA GATCCATTCC TGGGAGTGAA AGCTGCTGCA GCCGAGCTGG
     5461 AACGCAGATA CCCTGGTACA AGACTGGCAT GGCTGGCTGT CAGAGCTGAA GCACCATCTC
     5521 AGGTCCGACT GATGGACGTG GTGTCCAAGA AACACCCCGT GGACACCCTG TTCTTTCTGA
     5581 CCACCGTGTG GACAAGACCT GGCCCCGAAG TGCTGAACCG GTGCAGAATG AATGCCATCA
     5641 GCGGCTGGCA GGCATTTTTC CCAGTGCATT TTCAGGAGTT CAACCCCGCC CTGAGCCCAC
     5701 AGAGATCTCC ACCTGGACCT CCAGGCGCAG GACCTGATCC TCCTTCACCT CCTGGCGCTG
     5761 ATCCATCTAG AGGCGCTCCA ATCGGCGGCA GATTCGACAG ACAGGCCAGC GCCGAGGGAT
     5821 GCTTCTACAA TGCTGATTAC CTGGCCGCCA GAGCTAGACT CGCTGGCGAA CTCGCAGGAC
     5881 AGGAAGAGGA AGAAGCCCTC GAAGGCCTGG AAGTGATGGA TGTGTTCCTG AGGTTCAGCG
     5941 GACTGCACCT GTTTAGAGCC GTGGAACCTG GCCTCGTGCA GAAGTTCAGC CTGAGAGATT
     6001 GCTCCCCCAG ACTGAGCGAG GAACTGTACC ACAGATGCCG GCTGAGCAAC CTGGAAGGAC
     6061 TGGGAGGTAG AGCACAGCTG GCTATGGCCC TGTTTGAGCA GGAACAGGCT AACAGCACCT
     6121 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATAGCTTGGA TCCAATCAAC CTCTGGATTA
     6181 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG
     6241 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC
     6301 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA
     6361 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC
     6421 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT
     6481 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC
     6541 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG
     6601 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC
     6661 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGAGATC TGCCTCGACT
     6721 GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG
     6781 GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG
     6841 AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
     6901 GAAGACAATA GCAGGCATGC TGGGGACTCG AGTTAAGGGC GAATTGCCGA TTAGGATCTT
     6961 CCTAGAGCAT GGCTACGTAG ATAAGTAGCA TGGCGGGTTA ATCATTAACT ACAAGGAACC
     7021 CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG
     7081 ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG
     7141 CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT AGGCCTTGAC GGCCTTCCGC CAATTCGCCC
     7201 TATAGTGAGT CGTATTACGT CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA
     7261 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG
     7321 TAATAGCGAA GAGGCCCGCA CCGAAACGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
     7381 AATGGGAGCG CCCTGTAGCG GCCACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA
     7441 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC
     7501 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAG
//

